This book is a comprehensive overview of bioluminescence and chemiluminescence, from the basic principles and the most common luminescent systems to their applications in different fields -from analytical chemistry to biotechnology, miniaturized analytical devices, life sciences (whole-cell biosensors, bioluminescence molecular imaging) and multiplexed assays. Written by an expert, who for many years has been involved in the development of bio-and chemiluminescent (bio) analytical methods, this book documents the latest research in luminescence, thus representing the most updated reference text for people interested in the field. It is suitable not only for researchers currently employing detection techniques in their research activity, but also for those approaching the subject for the first time.