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How Enzymes Work de Haruo Suzuki, 9789814463928, QUÍMICA | FÍSICA, bioquímica | biofísica, Taylor and Francis, Inglés

How Enzymes Work

Haruo Suzuki

EAN9789814463928

TématicaQuímica, Física

SubtématicaBioquímica, Biofísica

EditorialTaylor and Francis

IdiomaInglés

FormatoCartoné   Año de publicación2015

 
For a long time, enzymes have been studied by measuring their activity, which has led to the advancement of "enzyme kinetics." In recent years, the mechanism of enzyme reaction has been explained in detail on the basis of the 3D structure. Genetic engineering and the 3D structural analysis of enzymes contribute to these advancements in enzymology. This book starts with an introduction to various enzymes to show how interesting enzymes are, which is followed by historical kinetic studies on enzymes and the overall and rapid-reaction kinetics. The subsequent topics describe the basics of protein structure, the control of enzyme activity, and the purification of enzymes. A case on the kinetic and structural studies of l-phenylalanine oxidase is also presented. There are many good books on enzyme kinetics, but few describe their kinetic and structural aspects. This book deals with both and contains many references that can be good sources for further reading. It is handy and is especially helpful for beginners. A number of figures, including some with stereo expression, facilitate observing the 3D structure of enzymes.
Preface

Chapter One

Introduction

General properties of enzyme

Examples of enzyme

References

Chapter Two

Overall reaction kinetics

Road to the steady state kinetics

Demonstration of the enzyme-substrate complex

Meaning of steady state

Kinetic parameters

Problems

References

Chapter Three

Factors affecting on the enzyme activity

Enzyme

Substrate concentration

Inhibitor

Problems

References

Chapter Four

Effect of pH, temperature, and high pressure on the enzymatic activity

Effect of pH

Thermodynamics in the enzymatic reaction

Temperature dependence of the enzymatic reaction

Effect of pressure

Effect of temperature and pressure on a-chymotrypsin-catalyzed reaction

Problems

References

Chapter Five

Measurements of individual rate constants

Rapid mixing techniques

Analysis of the first-order reaction

Problems

References

Chapter Six

Structure of proteins

Amino Acids

Polypeptide and protein

Analysis of primary structure

Three dimensional structure

Tertiary and quaternary structures

Structural motif and loop

Problems

References

Chapter Seven

Active site structure

Active site and active center

Cofactor, coenzyme, prosthetic group

Search of active site

Examples of active site studies

Problems

References

Control of enzyme activity

Regulation by non-covalent interaction

Regulation by covalent Modification

Problems

References

Chapter Nine

Preparation of enzyme

Extraction of enzyme

Purification of enzyme

Purity analysis of enzyme

Problems

References

Chapter Ten

A case study: L-Phenylalanine ocidase (deaminating and decarboxylating)

Introduction

Preparation of PAO

Catalytic properties of PAO

Molecular Properties of PAO

Physiological role of PAO

Problems

References

Appendix

Derivation of the rate equation by King-Altman’s method

Physical constants

Conversion of units

Greek numbers and alphabet

Useful softwares and data banks

Genetic code

Solutions for problems

Index

PVP:  119,55 €

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