For a long time, enzymes have been studied by measuring their activity, which has led to the advancement of "enzyme kinetics." In recent years, the mechanism of enzyme reaction has been explained in detail on the basis of the 3D structure. Genetic engineering and the 3D structural analysis of enzymes contribute to these advancements in enzymology. This book starts with an introduction to various enzymes to show how interesting enzymes are, which is followed by historical kinetic studies on enzymes and the overall and rapid-reaction kinetics. The subsequent topics describe the basics of protein structure, the control of enzyme activity, and the purification of enzymes. A case on the kinetic and structural studies of l-phenylalanine oxidase is also presented. There are many good books on enzyme kinetics, but few describe their kinetic and structural aspects. This book deals with both and contains many references that can be good sources for further reading. It is handy and is especially helpful for beginners. A number of figures, including some with stereo expression, facilitate observing the 3D structure of enzymes.

Preface Chapter One Introduction General properties of enzyme Examples of enzyme References Chapter Two Overall reaction kinetics Road to the steady state kinetics Demonstration of the enzyme-substrate complex Meaning of steady state Kinetic parameters Problems References Chapter Three Factors affecting on the enzyme activity Enzyme Substrate concentration Inhibitor Problems References Chapter Four Effect of pH, temperature, and high pressure on the enzymatic activity Effect of pH Thermodynamics in the enzymatic reaction Temperature dependence of the enzymatic reaction Effect of pressure Effect of temperature and pressure on a-chymotrypsin-catalyzed reaction Problems References Chapter Five Measurements of individual rate constants Rapid mixing techniques Analysis of the first-order reaction Problems References Chapter Six Structure of proteins Amino Acids Polypeptide and protein Analysis of primary structure Three dimensional structure Tertiary and quaternary structures Structural motif and loop Problems References Chapter Seven Active site structure Active site and active center Cofactor, coenzyme, prosthetic group Search of active site Examples of active site studies Problems References Control of enzyme activity Regulation by non-covalent interaction Regulation by covalent Modification Problems References Chapter Nine Preparation of enzyme Extraction of enzyme Purification of enzyme Purity analysis of enzyme Problems References Chapter Ten A case study: L-Phenylalanine ocidase (deaminating and decarboxylating) Introduction Preparation of PAO Catalytic properties of PAO Molecular Properties of PAO Physiological role of PAO Problems References Appendix Derivation of the rate equation by King-Altman’s method Physical constants Conversion of units Greek numbers and alphabet Useful softwares and data banks Genetic code Solutions for problems Index